Recombinant Mouse IL33 (E. coli)

Cat. No.
Cat. No. Z200155
Name Recombinant Mouse IL33 (E. coli)
Description IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells. IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes. Moreover, it was found that both human and murine mast cells when stimulated in vitro with IL-33 produced a wide spectrum of cytokines and chemokines . In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.
Organism Mouse (M. musculus)
Unit quantity 10 µg
Family IL-1 Family
Aliases IL-33
Recombinant Mouse Interleukin-33 (IL33)
Gene Symbol IL33
Gene ID 77125
Accession Number Q8BVZ5
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 18 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant Interleukin-33 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.
Function The ED(50) was determined by the dose-dependent proliferation of murine D10S cells is ≤ 0.3 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200155
Print & Download Datasheet
There are no FAQs for this product yet!
There are no references for this product yet!
This product has no review yet.
Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg