Recombinant Mouse MCP-5 (CCL12) (E. coli)

Cat. No.

Z200385

Unit

Price

$92.00

Cat. No.	Z200385
Name	Recombinant Mouse MCP-5 (CCL12) (E. coli)
Description	MCP-5 (CCL12) is a chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. It is also a potent active monocyte chemokine that signals through CCR2. It is Involved in allergic inflammation and the host response to pathogens. Also, it may play a pivotal role during early stages of allergic lung inflammation.
Organism	Mouse (M. musculus)
Unit quantity	25 µg
Family	Chemokine-beta Family (CC)
Tag	Tag Free
Aliases	MCP-5, Scya12
Gene Symbol	CCL12
Gene ID	20293
Accession Number	Q62401
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	10 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant MCP-5 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.
Function	Determined by its ability to chemoattract human monocytes using a concentration range of 20-40 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200385

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.