Recombinant Mouse MIP-3 (CCL19)

Cat. No.

Z200455

Unit

Price

$320.00

Cat. No.	Z200455
Name	Recombinant Mouse MIP-3 (CCL19)
Description	The chemokine MIP-3 beta is a chemoattractant for T and B lymphocytes, dendritic cells, macrophage progenitor cells, and natural killer cells. It might, therefore, play an important role in the trafficking of T cells in the thymus and migration of T and B cells to secondary lymphoid organs. Furthermore, MIP-3 beta has been recently shown to mediate rapid adhesion of naive CD4(+) T lymphocytes to activated endothelial cells supporting the role of this chemokine in regulation of lymphocyte homing. MIP-3 beta acts through CC chemokine receptor 7 (CCR7). The chemokines Lkn-1 and MIP-3 alpha are also lymphocyte attractants, although compared with MIP- 3 beta, their promigratory effect on lymphocytes is less intensive and their chemotactic activity is more promiscuous. Lkn-1 attracts monocytes, lymphocytes, and eosinophils via the chemokine receptors CCR1 and CCR3. MIP-3 alpha exhibits promigratory effects on lymphocytes, neutrophils, and immature dendritic cells via the CCR6 receptor.
Organism	Mouse (M. musculus)
Unit quantity	20 µg
Family	Chemokine-beta Family (CC)
Tag	Tag Free
Aliases	CKb11, ELC, Gm2023, MIP3B, Scya19, exodus-3
Gene Symbol	CCL19
Gene ID	24047
Accession Number	O70460
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	10 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant CCL19 was lyophilized from a 0.2 μm filtered 20 mM PB,100 mM NaCl solution pH 7.5.
Function	Determined by its ability to chemoattract human dendritic cells using a concentration range of 10-50 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200455

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Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.