Recombinant Mouse SHH (E. coli)

Cat. No.
Z200615
Unit
Price
$110.00
Cat. No. Z200615
Name Recombinant Mouse SHH (E. coli)
Description Mouse SHH is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. Mouse SHH shares 99% homology with the human gene for SHH. Sonic HedgeHog is a protein that is vital in guiding the early embryo; it has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH binds to the patched receptor to activate the transcription of target genes. Sonic HedgeHog also regulates another factor, the gli oncogene. Recombinant Mouse SHH is a single, non-glycosylated polypeptide chain of 19.8 kDa.
Organism Mouse (M. musculus)
Unit quantity 25 µg
Family Hedgehog Family
Aliases Sonic hedgehog protein, HHG-1,
Gene Symbol SHH
Gene ID 20423
Accession Number Q62226
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 19.8 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered solution in phosphate buffer and NaCl
Function The ED50 of this protein, as measured by its ability to induce alkaline phosphatase production in mouse myoblast cell, was in the range of 500ng-3 ug/mL.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200615
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