Recombinant Mouse Stem Cell Factor (KITLG)

Cat. No.

Z200505

Unit

100 µg

Price

$960.00

Cat. No.	Z200505
Name	Recombinant Mouse Stem Cell Factor (KITLG)
Description	SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL-7, Epo and some other growth and differentiation factors. In combination with IL-7 SCF stimulates the proliferation of pre-B cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.
Organism	Mouse (M. musculus)
Unit quantity	100 µg
Family	Other Growth Factors/Cytokines
Tag	Tag Free
Aliases	Clo, Con, Gb, Kitlg, Mgf, SCF, SF, SLF, Sl, blz, contrasted
Gene Symbol	KITLG
Gene ID	17311
Accession Number	P20826
Source	CHO cells
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	18 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant Stem cell factor was lyophilized from a 0.2 μm filtered solution in 20mM Tris, 5% trehalose, pH 7.5.
Function	The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells to be < 2.0 ng/mL, corresponding to a specific activity of > 5.0 x 10^5 units/mg.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200505

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.