Recombinant Rat CNTF (E. coli)

Cat. No.
Z300035
Unit
Price
$89.00
Cat. No. Z300035
Name Recombinant Rat CNTF (E. coli)
Description CNTF is found predominantly in peripheral nerve tissues. The main source appears to be myelin-associated Schwann cells in peripheral nerves and astrocytes in the central nervous system. CNTF is present at very high concentrations within intraocular tissues that contain the same muscle cells innervated by ciliary ganglionic neurons in vivo. CNTF receptors are expressed exclusively in the nervous system and skeletal muscle. The alpha subunit of the receptor is anchored to the cell membrane by a glycosyl-phosphatidylinositol linkage. The other receptor subunit, known as gp130, is also a component of receptors for LIF, Oncostatin M, IL-6 and IL-11, and seems to be involved also in signaling for CT-1. CNTF induces the expression of genes encoding hepatic acute phase proteins such as Haptoglobin, Alpha-1-antichymotrypsin, Alpha-2-Macroglobulin and Beta-fibrinogen in human hepatoma cells (HepG2) and in primary rat hepatocytes with a time course and dose-response comparable with that of IL-6.
Organism Rat (R. norvegicus)
Unit quantity 20 µg
Family CNTF/CCN Families
Aliases CNTF
Recombinant Rat Ciliary Neurotrophic Factor (CNTF)
Gene Symbol CNTF
Gene ID 25707
Accession Number P20294
Source E. coli
Species Rat (R. norvegicus)
Appearance Lyophilized Powder
Molecular Weight 22 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant Rat Ciliary Neurotrophic Factor was lyophilized from a 0.2 μm filtered PBS pH 7.5.
Function The ED(50) was determined by the dose-dependent proliferation of human TF1 cells was found to be in the range of 2-10 ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300035
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