Recombinant Rat CNTF (E. coli)

Cat. No.

Z300035

Unit

Price

$92.00

Cat. No.	Z300035
Name	Recombinant Rat CNTF (E. coli)
Description	CNTF is found predominantly in peripheral nerve tissues. The main source appears to be myelin-associated Schwann cells in peripheral nerves and astrocytes in the central nervous system. CNTF is present at very high concentrations within intraocular tissues that contain the same muscle cells innervated by ciliary ganglionic neurons in vivo. CNTF receptors are expressed exclusively in the nervous system and skeletal muscle. The alpha subunit of the receptor is anchored to the cell membrane by a glycosyl-phosphatidylinositol linkage. The other receptor subunit, known as gp130, is also a component of receptors for LIF, Oncostatin M, IL-6 and IL-11, and seems to be involved also in signaling for CT-1. CNTF induces the expression of genes encoding hepatic acute phase proteins such as Haptoglobin, Alpha-1-antichymotrypsin, Alpha-2-Macroglobulin and Beta-fibrinogen in human hepatoma cells (HepG2) and in primary rat hepatocytes with a time course and dose-response comparable with that of IL-6.
Organism	Rat (R. norvegicus)
Unit quantity	20 µg
Family	CNTF/CCN Families
Tag	Tag Free
Aliases	MGC10870, Fbw9
Gene Symbol	CNTF
Gene ID	25707
Accession Number	P20294
Source	E. coli
Species	Rat (R. norvegicus)
Appearance	Lyophilized Powder
Molecular Weight	22 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant Rat Ciliary Neurotrophic Factor was lyophilized from a 0.2 μm filtered PBS pH 7.5.
Function	The ED(50) was determined by the dose-dependent proliferation of human TF1 cells was found to be in the range of 2-10 ng/ml.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300035

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.