Recombinant Rat CXCL1 (E. coli)

Cat. No.
Z300105
Unit
Price
$89.00
Cat. No. Z300105
Name Recombinant Rat CXCL1 (E. coli)
Description Growth regulated oncogene- alpha belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL1. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.
Organism Rat (R. norvegicus)
Unit quantity 25 µg
Family Chemokine-alpha Family (CXC)
Aliases C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, CINC-1, Platelet-derived growth factor-inducible protein KC, Cinc1, Gro, Scyb1
Recombinant Rat Growth-Regulated Alpha Protein (CXCL1)
Gene Symbol CXCL1
Gene ID 81503
Accession Number P14095
Source E. coli
Species Rat (R. norvegicus)
Appearance Lyophilized Powder
Molecular Weight 8 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS pH 7.4.
Function The ED(50) was determined by the dose-dependent proliferation of Ovcar3 cells and was found to be <0.05ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300105
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