Recombinant Rat IGF1 (E. coli)

Cat. No.
Z300055
Unit
Price
$126.00
Cat. No. Z300055
Name Recombinant Rat IGF1 (E. coli)
Description Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.
Organism Rat (R. norvegicus)
Unit quantity 50 µg
Family IGF/Insulin Family
Aliases Somatomedin, Insulin-like growth factor I, IGF-I, Igf1
Recombinant Rat Insulin-Like Growth Factor (IGF1)
Gene Symbol IGF1
Gene ID 24482
Accession Number P08025
Source E. coli
Species Rat (R. norvegicus)
Appearance Lyophilized Powder
Molecular Weight 8 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant Rat IGF1 was lyophilized from a 0.2 μm filtered PBS solution, pH 7.4.
Function The ED(50) was determined by a cell proliferation assay using FDC-P1 cells is ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1.0 x 10^6 units/mg.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300055
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