Recombinant Rat IL1B (E. coli)

Cat. No.

Z300075

Unit

Price

$92.00

Cat. No.	Z300075
Name	Recombinant Rat IL1B (E. coli)
Description	Monocytes are the main source of secreted IL-1. They express predominantly IL-1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T cells is expressed predominantly on T cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B cells, granulocytes, and macrophages. It is expressed predominantly on B cells and cells of the myelomonocytic lineage and is encoded by a separate gene.
Organism	Rat (R. norvegicus)
Unit quantity	10 µg
Family	IL-1 Family
Tag	Tag Free
Aliases	IL-1F2
Gene Symbol	IL1B
Gene ID	24494
Accession Number	Q63264
Source	E. coli
Species	Rat (R. norvegicus)
Appearance	Lyophilized Powder
Molecular Weight	17 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Rat IL-1 beta was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.
Function	The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤ 2.0 pg/mL, corresponding to a specific activity of ≥ 5.0 x 10^8 units/mg.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300075

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.