Recombinant Rat IL3 (E. coli)

Cat. No.
Z300205
Unit
5 µg
Price
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Cat. No. Z300205
Name Recombinant Rat IL3 (E. coli)
Description Rat IL3 is a pleiotrophic cytokine produced by activated T cells. IL3 functions via specific cell surface receptors to activate hematopoiesis by controlling the production, differentiation, and proliferation of granulocytes and monocytes. IL3 also affects the activity of mature mast cells, eosinophils, basophils, and macrophages. The high affinity IL3 receptor is composed of an alpha and beta subunit, where the IL3R alpha is a member of the cytokine receptor super family and binds to IL3 with low affinity. The beta subunit does not bind any cytokine but binds to the alpha subunit of the IL3, IL5 and GM-CSF receptors with high affinity. The receptors for IL3 can be found in bone marrow, macrophages, mast cells, megakaryocytes, and other similar cells.
Organism Rat (R. norvegicus)
Unit quantity 5 µg
Family Other Interleukins
Aliases Hematopoietic growth factor, mast cell growth factor, multipotential colony-stimulating factor, P-cell-stimulating factor
Gene Symbol IL3
Accession Number P04823
Source E. coli
Species Rat (R. norvegicus)
Appearance Lyophilized Powder
Molecular Weight 15.9 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered solution in PBS
Function The ED50 is 1.0-5.0 ng/mL as determined by a cell proliferation assay
Reconstitution Reconstitute in sterile PBS at 100 µg/mL
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300205
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