SEC Component FLAG-AF9 Stable Expressing HeLa S3 Cell Line

CAT.NOUNITPRICE
T63881x106 cells / 1.0 ml
$0.00

Specifications


DescriptionHeLa S3 cells are derived from the parental HeLa cell line. To establish this stable cell line; mCAT1 was transfected into the HeLa S3 cells using FuGene HD. The SEC component FLAG-AF9 was then transduced into the HeLa-S3-mCAT1 cells via Ecotropic retrovirus system. This cell line is G418 and hygromycin resistant and can be used in DNA transcription studies.
SKUT6388
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemCervix
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Seeding Density40,000 - 50,000 cells/cm2
Population Doubling Time24 - 34 hours
Donor GenderFemale
Donor GenderFemale
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileG418 resistant (ecotropic retroviral infection with mCAT1) Hygromycin resistant
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) PCR; 2) Immunoblotting
Documents


Supporting Protocol
MSDS

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