SIRT7 Stable Knockdown H1299 Cell Line
|T6165||1x106 cells / 1.0 ml|
|Description||The sirtuin family of proteins have a diverse range of biological functions accomplished by the deacetylation of their targets. Derived from H1299 cells; this Sirt7 knockdown cell line was displays lentiviral-driven stable expression shRNA scramble sequences and a Sirt7 specific shRNA. These cells would be suitable for use in the study of the functions of Sirt7 and the sirtuin family of proteins|
|Species||Human (H. sapiens)|
For Research Use Only
|Seeding Density||10,000 - 20,000 cells/cm2|
|Population Doubling Time||43 - 53 hours|
|Donor Age||43 years old|
|Cell Type||Drug Discovery Cell Lines|
Puromycin resistance at the concentration of 5 ug/mL. The cells stably express the short hairpin RNA (shRNA) scramble sequence: 5'-CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTT AGG-3' and the Sirt7 specific shRNA 5'- CCGGGTCCAGCCTGAAGGTTCTAAACTCGAGTTTAGAACCTTCAGGCTGGACTTTTTG-3'.
1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
1) Immunofluorescence; 2) DNA analysis and qPCR; 3) AgNOR staining
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