Stable CYP24A1 Expressing LS180 Cell Line

CAT.NOUNITPRICE
T61121x106 cells / 1.0 ml
$0.00

Specifications


DescriptionHuman Caucasian colon adenocarcinoma cell line LS 180 was transfected with reporter plasmid CYP24_minP-pNL2.1[Nluc/Hygro] to generate Stable CYP24A1 Expressing LS180 Cell Line. Stable CYP24A1 Expressing LS180 Cell Line contains much of the CYP24A1 promoter and therefore, can be used for study of transcriptional regulation of CYP24A1. This cell line allows for measurement of Vitamin D receptor transcriptional activity. This cell line can be used to profile VDR transcriptional activity in response to analogues of vitamin D, and intermediates in vitamin D2 and vitamin D3 metabolic pathways.
SKUT6112
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemDigestive
Growth PropertiesAdherent
Cell MorphologyEpithelial
Unit quantity1x106 cells / 1.0 ml
Cell TypeDrug Discovery Cell Lines
Expression Profile

Luciferase

Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255), 4 mM L-glutamine (G275), 1x non essential amino acids, and 1 mM Sodium Pyruvate Solution (TM057).
Carbon dioxide (CO2): 5%, Temperature: 37.0
QC

1) Test of functionality after cryopreservation; 2) Test of long-term luciferase inducibility; 3) Assay performance

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

Documents


Supporting Protocol
MSDS

    QC

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