Stable ΔN-IκBα Expressing HOS-G (HOS-G/ΔN-IκBα) Cell Line

CAT.NOUNITPRICE
T61081x106 cells / 1.0 ml
$0.00

Specifications


DescriptionHuman Osetosarcoma (HOS) cells were transfected with a Lentivirus vector to stably express a degradation resistant I-κBα (?N-I-κBα) under the control of a SV40 promoter. Successfully transfected cells were selected for puromycin resistance. As a result of the constitutive repression of NF-κB by the degradation resistant inhibitor ?N-I-κBα, these cells are resistant to senescence caused by infection with human T-lymphotropic virus type 1 (HTLV-1). HOS-G/ΔN-IκBα line can be productively infected by HTLV-1 and can spread HTLV-1 to susceptible cells. This line also contains a reporter cassette consisting of 18 copies of Tax-inducible HTLV-1 21bp repeat, the HTLV-1 TATA element, the complete R region, and part of the U5 sequence fused with enhanced green fluorescence protein (eGFP). As a result of this reporter element the expression of eGFP is a positive indicator of successful HTLV-1 infection in cells. It is suggested that this cell line can be used to study of HTLV-1 replication in cell culture.
SKUT6108
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemSkeletal
Donor Age
Cell MorphologyBipolar
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Cell TypeDrug Discovery Cells
Expression Profile

δN-I-κBα , eGFP

Propagation RequirementsThe base medium for this cell line is Prigrow III available at abm, Cat. No. TM003 . To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 2mM L-glutamine (G275), Penicillin/Streptomycin (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0
QC

1) Verified the expression of GFP upon HTLV-1 infection via imaging (Figure 1). 2) Verified the expression of δN-IκBα in Western blot (Figure 1). 3) Verified that cell line continues to propagate after HTLV-1 infection (Figure 2). 4) Confirmed the expression of viral genes Tax, Rex and Gag through Western Blot analysis ( HTLV-1 proteins) (Figure 3A). 5) Verified viral spread to B-cells through Electron microscopy (Figure 3B). 6) Showed cells could spread from one HOS line to another as well as to T-cell reporter line by imaging RFP expression and measuring Luciferase activity of the T-cell reporter line (Figure 5).

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorThe Henry M. Jackson Foundation for the Advancement of Military Medicine
Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      There are no FAQs for this product yet!
      References


      There are no references for this product yet!