AAV Packaging Mix (Serotype 4)
Cat. No.
AAV1004
Unit
400 µl
Price
$395.00
| Cat. No. | AAV1004 | ||||||
| Name | AAV Packaging Mix (Serotype 4) | ||||||
| Unit | 400 µl | ||||||
| Description |
abm’s AAV Packaging Mix (Serotype 4) offers a high-efficiency solution for recombinant adeno-associated virus production. This second generation AAV system requires three plasmids for viral particle production: The ready-to-use AAV Packaging Plasmid Mix combines optimized Packaging and Helper Plasmids for high viral titers, enhanced biosafety and reliable gene delivery performance.
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| Applications | |||||||
| Storage Condition | -20°C or below. | ||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. AAV1004 |
Datasheet
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MSDS
| Is it possible to propagate the packaging plasmids from this product mix? | |
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No, it is not possible to grow up the plasmids from the product.
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| What is the concentration of your packaging plasmid mixes? | |
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The packaging plasmid mixes are offered at 0.5 µg/µl.
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| Do I need a Helper virus to generate AAV particles? | |
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No. All of abm's AAV Packaging Mixes can generate AAV particles without the need of a Helper virus.
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| What is the maximum AAV cargo size on my Transfer Plasmid? | |
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The functional packaging limit is approximately ~4.4 kb excluding ITRs on the Transfer Plasmid. Exceeding this often results in truncated genomes and reduced yield.
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| How many AAV can I package using abm's AAV Packaging Mixes? | |
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Using 15cm vessels, it is possible to package 3 AAV using abm's AAV Packaging Mixes.
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| What cell line should I use for AAV production? | |
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We recommend using HEK293T cell line due to its high transfection efficiency and virus yield.
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