Immortalized Human Endometrial Stromal Cells (HESC)

T05331x106 cells / 1.0 ml



Decidualization of the endometrium, mainly induced by progesterone, is a process of endometrial remodelling where the endometrial stromal cells differentiate into secretory decidual cells in preparation for pregnancy. Decidualized endometrial stromal cells are characterized by release of prolactin (PRL), insulin-like growth factor binding protein-1 (IGFBP-1), tissue factor (TF), plasminogen activator inhibitor-1 (PAI-1), and expression of basal lamina components such as laminin, collagen IV, and fibronectin.

Immortalized Human Endometrial Stromal Cells (HESC) are unique in that 1) they display normal karyotype and 2) they respond to hormone stimulation and retain the morphological pattern and biochemical endpoints of decidualization after treatment of estradiol and medroxyprogesterone, making this cell line a valuable tool in endometrium homeostasis and female reproductive studies.

Note: The cells were collected from the endometrium of women who underwent hysterectomy, thus there is no specific age as the cells are pooled together to generate T0533. 

SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemReproductive
Donor GenderFemale
Donor AgeAdult
Donor DiseaseNon-malignant myomas (Fibroids).
Growth PropertiesAdherent
Cell MorphologyFibroblast-like
Seeding Density10,000 – 20,000 cells/cm2 Recommended split ratio of 1:3 to 1:6
Population Doubling Time32-42 hours
Immortalization MethodSerial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Cell TypeImmortalized Cells
Expression Profile

IGFBP-1, PRL, TF, PAI-1 at decidualization endpoints

Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: charcoal-stripped fetal bovine serum to a final concentration of 10%, L-glutamine (G275) to a final concentration of 1%,  and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Stimulation of HESC can be done using 10-8 M estradiol (E2) and/or 10-7 M medroxyprogesterone acetate (MPA).
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

1) TRAPezeELISA was used to detect the telomerase activity in the immortalized cells; 2) ELISA and RT-PCR were performed to detect IGFBP-1, FN, PRL and PAI-1 secretion and mRNA expression; 3) Western blot and densitometry were conducted to determine the expression of TF and Fas/Fas ligand; 4) 800 ng puromycin was used for selection.


1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."


I want to make sure these cells express my gene of interest before I decide to buy the cell line. Can you provide a sample so this can be tested?
We do not carry out downstream characterization or gene expression profiling of our cell lines. To facilitate your preliminary experiments we can provide an RNA extraction (0.5ug total RNA) or cell lysate (100ug/100ul provided in 62.5mM Tris‐HCl, 2% SDS, 10% Glycerol, 50mM DTT, 0.01% w/v Bromophenol Blue) for any of our immortalized cell lines for a small fee. Please inquire directly for more information. The lead time will be around 2 weeks from the time of placing an order (if the item is in stock).
How often do I need to change the media?
The media should be changed every 2-3 days.
Why do these cells need bio safety level II?
In order to be more cautious, we follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site if required.
Do you sell ECM coated T75 flasks?
Yes we can provide a coating service. Please inquire with [email protected]
What can I coat a larger dish to subculture?
We also offer applied extracellular matrix (collagen type I) in liquid form, for the coating of larger flasks and other required plasticware:
How long can I store frozen vials for?
Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen indefinitely without affecting their recovery.
Should the cap of the flask be changed before starting the cell culturing step?
No, there is no need in sterile biosafety cabinets unless it has contacted any non-sterile condition (e.g. touching the contaminated tip, etc.).
What is the recommended storage temperature?
In general, if you received: Live cells: acclimatize for 3-4 hrs at at the recommended conditions stated for the cell line under the propagation section, and then change media afterwards. Frozen cells: Immediately place cells in liquid nitrogen; -180C.
How is cell density crucial for drug selection?
If antibiotic selection is applicable to the target cells, we suggest getting rid of all the background cells so that the cell density is kept lower (even 20-30%). However, once the clones are selected by clonal dilution, we don't need the drug to still be present. If needed, the cell density should be towards the higher end since cells are already selected. Any primary cells still present will be depleted as a result of senescence and the cell population that remains will be resistant to the specific antibiotic.
My cells are not detaching, what method do you recommend to trypsinize the cells?
1. Incubate the coated plate containing trypsin solution at recommended temperature indicated in the propagation section for 3-5 min till the cells round up, monitoring from time to time under microscope. 2. Diluting G422 (1:1) with PBS and coating for lesser time. Sometimes the collagen content in G422 is higher and thus make stronger bonding with cells. 3. You can try reducing the incubation time as well for coating the plate to make a thinner layer.
Why is it important to determine the optimal seeding density?
The seeding density we recommend is for when cells are plated to a new vessel. The optimal seeding density should allow cells to attach to the surface and have room to proliferate. If you seed too little, cells may not attach well to the surface (for adherent cells). Seeding density is important as many cells (adherent or suspension cells) need to be in close proximity for better growth. Cell-cell interactions allow cells to communicate with each other in response to changes in their microenvironment. This ability to send and receive signals is essential for the survival of the cell. In other cases, if the seeding density is too low, cells may attach but a retardation in cell growth is observed. If you seed too high, the cells will attach but there is insufficient room for further proliferation and they will stop replicating.

  • Krikun, G et al. "A novel immortalized human endometrial stromal cell line with normal progestational response" Endocrinology 145:2291-2296 (2004). PubMed: 14726435.
  • Krikun, G et al. "Endometrial angiopoietin expression and modulation by thrombin and steroid hormones: a mechanism for abnormal angiogenesis following long-term progestin-only contraception" Am J Pathol 164:2101-2107 (2004). PubMed: 15161644.
  • Ono, YJ et al. "Decorin induced by progesterone plays a crucial role in suppressing endometriosis" J Endocrinol 223(2):203-216 (2014). DOI: 10.1530/JOE-14-0393.
  • Nothnick, WB et al. "miR-451 deficiency is associated with altered endometrial fibrinogen alpha chain expression and reduced endometriotic implant establishment in an experimental mouse model" PLoS One 9(6):e100336 (2014). DOI: 10.1371/journal.pone.0100336.
  • Frohlich, LF et al. "Epigenetic silencing of apoptosis-inducing gene expression can be efficiently overcome by combined SAHA and TRAIL treatment in uterine sarcoma cells" PLoS One 9(3):e91558 (2014). DOI: 10.1371/journal.pone.0091558.
  • Zhang, C et al. "Liver receptor homolog-1 is essential for pregnancy" Nat Med 19(8):1061-1066 (2013). DOI: 10.1038/nm.3192.
  • Daftary, GS et al. "A novel role of the Sp/KLF transcription factor KLF11 in arresting progression of endometriosis" PLoS One 8(3):e60165 (2013). DOI: 10.1371/journal.pone.0060165.
  • Panagopoulos, I et al. "Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs" Oncol Lett 1(6):947-950 (2010). PubMed: 22870092.
  • Han, SJ et al. "A new isoform of steroid receptor coactivator-1 is crucial for pathogenic progression of endometriosis" Nat Med 2012:18(7) (2012). DOI: 10.1038/nm.2826.
  • Dellinger, TH et al. "Expression of the Wnt antagonist Dickkopf-3 is associated with prognostic clinicopathologic characteristics and impairs proliferation and invasion in endometrial cancer" Gynecol Oncol 126(2):259-267 (2012). PubMed: 22555103.
  • Toh, MF et al. "Biological characterization of non-steroidal progestins from botanicals used for women's health" Steroids 77(7):765-773 (2012). DOI: 10.1016/j.steroids.2012.03.013.
  • Colon-Diaz, M et al. " HDAC1 and HDAC2 are differentially expressed in endometriosis" Reprod Sci 19(5):483-492 (2012). DOI: 10.1177/1933719111432870.
  • Schutte, SC et al. "A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation" Fertil Steril 97(4):997-1003 (2012). PubMed: 22306710.
  • Saleh, L et al. "Evaluation of human first trimester decidual and telomerase-transformed endometrial stromal cells as model systems of in vitro decidualization" Reprod Biol Endocrinol 9:155 (2011). PubMed: 22151839.
  • Holmberg, JC et al. "An in vitro model for the study of human implantation" Am J Reprod Immunol 67(2):169-178 (2012). PubMed: 22151560.
  • Huyen, DV et al. "Evidence for a conserved function of heart and neural crest derivatives expressed transcript 2 in mouse and human decidualization" Reproduction 142(2):353-368 (2011). PubMed: 21527398.
  • Zhou, Y et al. "Cigarette smoke increases progesterone receptor and homeobox A10 expression in human endometrium and endometrial cells: a potential role in the decreased prevalence of endometrial pathology in smokers" Biol Reprod 84(6):1242-1247 (2011). PubMed: 21325691.
  • Lisboa, FA et al. "Pregnancy-specific glycoprotein 1 induces endothelial tubulogenesis through interaction with cell surface proteoglycans" J Biol Chem 286(9):7577-7586 (2011). PubMed: 21193412.
  • Wu, J et al. "Retinoic acid stimulation of VEGF secretion from human endometrial stromal cells is mediated by production of reactive oxygen species" J Physiol 589(Pt 4):863-875 (2011). PubMed: 21173077.
  • Rodgers , AK et al. "nhibition of CD44 N- and O-linked glycosylation decreases endometrial cell lines attachment to peritoneal mesothelial cells" Fertil Steril 95(2):825-825 (2011). DOI: 10.1016/j.fertnstert.2010.09.005.
  • Doherty, LF et al. "Regulation of tryptophan 2,3-dioxygenase by HOXA10 enhances embryo viability through serotonin signaling" Am J Physiol Endocrinol Metab 300(1):E86-E93 (2011). PubMed: 20959529.
  • Du, H et al. "HOXA10 inhibits Kruppel-like factor 9 expression in the human endometrial epithelium" Biol Reprod 83(2):205-211 (2010). PubMed: 20463357.
  • Pabona, JM et al. "Functional differentiation of uterine stromal cells involves cross-regulation between bone morphogenetic protein 2 and Kruppel-like factor (KLF) family members KLF9 and KLF13" Endocrinology 151(7):3396-3406 (2010). DOI: 10.1210/en.2009-1370.
  • Shu, FJ et al. "The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses" J Steroid Biochem Mol Biol 120(4-5):172-179 (2010). PubMed: 20403436.
  • Peng, B et al. "Phagocytosis of apoptotic trophoblast cells by human endometrial endothelial cells induces proinflammatory cytokine production" Am J Reprod Immunol 64(1):12-9 (2010). PubMed: 20219062.
  • Mason, CE et al. "Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements" Nucleic Acids Res 38(7):2355-2355 (2010). DOI: 10.1093/nar/gkp1188.
  • Sidell, N et al. "Retinoic acid is a cofactor for translational regulation of vascular endothelial growth factor in human endometrial stromal cells" Mol Endocrinol 24(1):148-160 (2010). PubMed: 19910455.
  • Du, H et al. "3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells" Reproduction 139(1):237-245 (2010). PubMed: 19778996.
  • Straszewski-Chavez, SL et al. "The isolation and characterization of a novel telomerase immortalized first trimester trophoblast cell line, Swan 71" Placenta 30(11):939-948 (2009). PubMed: 19766308.
  • Alvero, AB et al. "Stem-like ovarian cancer cells can serve as tumor vascular progenitors" Stem cells 27(10):2405-2413 (2009). PubMed: 19658191.
  • Andikyan, V et al. "WT1 represses HOX gene expression in the regulation of gynaecologic tumour histologic type" J Cell Mol Med 13(11-12):4522-4531 (2009). DOI: 10.1111/j.1582-4934.2008.00574.x.
  • Wang, C et al. "Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells" PLoS One 3(10):e3523 (2008). PubMed: 18949048.
  • Pabona, JM et al. "Nuclear receptor co-regulator Krüppel-like factor 9 and prohibitin 2 expression in estrogen-induced epithelial cell proliferation in the mouse uterus" J Endocrinol 200(1):63-73 (2009). DOI: 10.1677/JOE-08-0383.
  • Laws, MJ et al. "Gap junction communication between uterine stromal cells plays a critical role in pregnancy-associated neovascularization and embryo survival" Development 135(15):2659-2659 (2008). PubMed: 18599509.
  • Belton, RJ et al. "Basigin-2 is a cell surface receptor for soluble basigin ligand" J Biol Chem 283(26):17805-17814 (2008). PubMed: 18434307.
  • Gonzalezl, M et al. "Expansion of human trophoblastic spheroids is promoted by decidualized endometrial stromal cells and enhanced by heparin-binding epidermal growth factor-like growth factor and interleukin-1β" Mol Hum Reprod 17(7):421-433 (2011). DOI: 10.1093/molehr/gar015.
  • Hrzenjak, A et al. "SAHA induces caspase-independent, autophagic cell death of endometrial stromal sarcoma cells by influencing the mTOR pathway" J Pathol 216(4):495-504 (2008). DOI: 10.1002/path.2434.
  • Rivero , R et al. "Protein expression of PKCZ (Protein Kinase C Zeta), Munc18c, and Syntaxin-4 in the insulin pathway in endometria of patients with polycystic ovary syndrome (PCOS) " Reprod Biol Endocrinol 10:17 (2012). DOI: 10.1186/1477-7827-10-17.
  • Subramaniam, KS et al. "Cancer-Associated Fibroblasts Promote Proliferation of Endometrial Cancer Cells" PLoS One 8(7):e68923 (2013). DOI: 10.1371/journal.pone.0068923.
  • Gellersen, B et al. "Human Endometrial Stromal Cell-Trophoblast Interactions: Mutual Stimulation of Chemotactic Migration and Promigratory Roles of Cell Surface Molecules CD82 and CEACAM1" Biol Reprod 88(3):80 (2013). DOI: 10.1095/biolreprod.112.106724.
  • Lu, Y et al. "Modulation of the Expression of the Transcription Factors T-Bet and GATA-3 in Immortalized Human Endometrial Stromal Cells (HESCs) by Sex Steroid Hormones and cAMP" Reprod Sci 20(6):699-709 (2013). PubMed: 23308012.
  • Gellersen, B et al. "9. Human endometrial stromal cell-trophoblast interactions: mutual stimulation of chemotactic migration and promigratory roles of cell surface molecules CD82 and CEACAM1" Biology of Reproduction 88(3):Article 80, 1-13 (2013). DOI: 10.1095/biolreprod.112.106724.
  • Wagner, GP et al. "A model based criterion for gene expression calls using RNA-seq data" Theory Biosci 132(3):159-164 (2013). PubMed: 23615947.
  • Frohlich, LF et al. "Epigenetic Silencing of Apoptosis-Inducing Gene Expression Can Be Efficiently Overcome by Combined SAHA and TRAIL Treatment in Uterine Sarcoma Cells" PLoS One 9(3):e91558 (2014). DOI: 10.1371/journal.pone.0091558.
  • Emera, D et al. "Convergent evolution of endometrial prolactin expression in primates, mice, and elephants through the independent recruitment of transposable elements" Mol Biol Evol 29(1):239-247 (2012). DOI: 10.1093/molbev/msr189.
  • Korir, ML et al. "Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells" Infect Immun 82(11):4587-4595 (2014). DOI: 10.1128/IAI.02181-14.
  • Daftary, GS et al. "A Novel Role of the Sp/KLF Transcription Factor KLF11 in Arresting Progression of Endometriosis" PLoS One 8(3):e60165 (2013). DOI: 10.1371/journal.pone.0060165.
  • Plaza-Parrochia, F et al. "The role of androst-5-ene-3β,17β-diol (androstenediol) in cell proliferation in endometrium of women with polycystic ovary syndrome" Steroids 89:11-19 (2014). PubMed: 25065586.
  • Huang, Zhu et al. "Cyclic adenosine monophosphate-induced argininosuccinate synthase 1 expression is essential during mouse decidualization" Mol Cell Endocrinol 388(1-2):20-31 (2014). PubMed: 24556046.
  • Brayer, KJ et al. "Evolution of a derived protein–protein interaction between HoxA11 and Foxo1a in mammals caused by changes in intramolecular regulation" PNAS 108(32):E414-E420 (2011). DOI: 10.1073/pnas.1100990108 .
  • Zhang, C et al. "Liver receptor homolog-1 is essential for pregnancy" Nat Med 19(8):1061-1066 (2013). PubMed: 23817023 .
  • Emera, D et al. "Transformation of a transposon into a derived prolactin promoter with function during human pregnancy" Proc Natl Acad Sci USA 109(28):11246-11251 (2012). PubMed: 22733751.
  • Lynch, VJ et al. "Transposon-mediated rewiring of gene regulatory networks contributed to the evolution of pregnancy in mammals" Nat Genet 43(11):1154-1159 (2011). PubMed: 21946353.
  • Lynch, VJ et al. "HoxA-11 and FOXO1A Cooperate to Regulate Decidual Prolactin Expression: Towards Inferring the Core Transcriptional Regulators of Decidual Genes" PLoS One 4(9):e6845 (2009). DOI: 10.1371/journal.pone.0006845.
  • Tang, W., Chen, O., Yao, F., & Cui, L. "miR‑455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide" Molecular medicine reports. : (2019).