Immortalized Human Endometrial Stromal Cells (HESC)

Cat. No.
T0533
Unit
1x106 cells / 1.0 ml
Price
Inquiry

Worried about losing your cells due to contamination issues, thawing or growth difficulties or even a random freezer breakdown?

Enjoy peace of mind knowing that you can be covered under abm’s Cell Line Insurance

Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

Cat. No. T0533
Name Immortalized Human Endometrial Stromal Cells (HESC)
Description

Decidualization of the endometrium, mainly induced by progesterone, is a process of endometrial remodelling where the endometrial stromal cells differentiate into secretory decidual cells in preparation for pregnancy. Decidualized endometrial stromal cells are characterized by release of prolactin (PRL), insulin-like growth factor binding protein-1 (IGFBP-1), tissue factor (TF), plasminogen activator inhibitor-1 (PAI-1), and expression of basal lamina components such as laminin, collagen IV, and fibronectin.

Immortalized Human Endometrial Stromal Cells (HESC) are unique in that 1) they display normal karyotype and 2) they respond to hormone stimulation and retain the morphological pattern and biochemical endpoints of decidualization after treatment of estradiol and medroxyprogesterone, making this cell line a valuable tool in endometrium homeostasis and female reproductive studies.

Note: The cells were collected from the endometrium of women who underwent hysterectomy, thus there is no specific age as the cells are pooled together to generate this cell line. 


Organism Human (H. sapiens)
Tissue Endometrium
Donor History Female, Adult, Non-malignant myomas (Fibroids)
Growth Properties Adherent, fibroblast-like
Cell Type Immortalized Cells
Unit 1x106 cells / 1.0 ml
Storage Condition Vapor phase of liquid nitrogen, or below -130°C.
Shipping Conditions Ship with dry ice.
Product Format Frozen
Intended Use This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
BioSafety II
Certificate of Analysis For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.
Growth Conditions

Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 2 mM L-glutamine (G275) + 10% charcoal-stripped FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂

Unpacking and Storage Instructions

1. Visually examine the packaging containers for signs of leakage or breakage.

2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use.

To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.


Thawing Protocol

1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.

2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.

3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.

4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.

5. Incubate the cells at the recommended conditions.

Subculture Protocol

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.

6. Incubate the cells at the recommended conditions.

Cryopreservation

Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Seeding Density (cells/cm2) 10,000 – 20,000
Split Ratio 1:3 to 1:6
Population Doubling Time (h) 32 - 42
Immortalization Method

Retrovial transfection with hTERT

Expression

IGFBP-1, PRL, TF, PAI-1 at decidualization endpoints, Puromycin-resistance

STR Profiling

D5S818 : 8,8

D13S317 : 12,13

D7S820 : 10,10

D16S539 : 12,14

VWA : 16,18

TH01 : 7,8

AMEL : X,X

TPOX : 9,11

CSF1PO : 10,12

D12S391 : 18,20

FGA : 20,21

D2S1338 : 17,17

D21S11 : 30,31.2

D18S51 : 15,16

D8S1179 : 14,16

D3S1358 : 16,17

D6S1043 : 11,17

PENTAE : 5,7

D19S433 : 13,14

PENTAD : 10,11

D1S1656 : 13,14

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0533.
Warranty abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Disclaimer
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Depositor Yale
Application Research Use Only.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T0533
Print & Download Datasheet
  • Krikun, G., Mor, G., Alvero, A., Guller, S., Schatz, F., Sapi, E., Rahman, M., Caze, R., Qumsiyeh, M., & Lockwood, C. J. (2004). A novel immortalized human endometrial stromal cell line with normal progestational response. Endocrinology, 145(5), 2291–2296. https://doi.org/10.1210/en.2003-1606


    Tang, W., Chen, O., Yao, F., & Cui, L. (2019). miR‑455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide. Molecular medicine reports.


    Alauddin, M., Okumura, T., Rajaxavier, J., Khozooei, S., Pöschel, S., Takeda, S., … Salker, M. S. (2020). Gut Bacterial Metabolite Urolithin A Decreases Actin Polymerization and Migration in Cancer Cells. Molecular Nutrition & Food Research, 64(7), 1900390. doi:10.1002/mnfr.201900390


    Alauddin, M., Salker, M. S., Umbach, A. T., Rajaxavier, J., Okumura, T., Singh, Y., ... & Lang, F. (2020). Annexin A7 regulates endometrial receptivity. Frontiers in cell and developmental biology, 8, 770.


    Lin, Y., Kojima, S., Ishikawa, A., Matsushita, H., Takeuchi, Y., Mori, Y., ... & Wakatsuki, A. (2023). Inhibition of MLCK‑mediated migration and invasion in human endometriosis stromal cells by NF‑κB inhibitor DHMEQ. Molecular Medicine Reports, 28(2), 1-10.

This product has no review yet.
Other Cell Lines
SEARCH RESULT