KDR Stable Ba/F3 Cell Line (IL-3 Dependent)

CAT.NOUNITPRICE
T60271x106 cells / 1.0 ml
$0.00

Specifications


Description

Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The KDR Stable Ba/F3 Cell Line expresses activated KDR tyrosine kinase that is linked to a GFP reporter (GFP-ETV6-KDR). KDR belongs to the VEGF receptor tyrosine kinase family consisting of proteins which bind to vascular endothelial growth factors. These proteins play an important role in forming the vascular system during embryogenesis and beyond, in conjunction with maintenance. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signaling and trafficking of this receptor are regulated by multiple factors. Mutations of its gene are implicated in infantile capillary hemangiomas.

SKUT6027
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemBlood
Growth PropertiesSuspension
Cell MorphologyRound
Seeding Density100,000 - 300,000 cells/ml
Population Doubling Time16 - 26 hours
Applications

For Research Use Only

Mammalian Selection MarkerPuromycin
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
Cell TypeDrug Discovery Cell Lines
Propagation Requirements

The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 10 ng/ml mouse IL-3 (Z200145), 1mM HEPES (Corning), 1% GlutaMAX (Gibco), 1% Non-essential Amino Acids (Corning), 1mM Sodium Pyruvate Solution (TM057) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Once the cells have recovered from thaw 1 µg/ml Puromycin (G264) can be added.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Use 30% FBS for the initial recovery of these cells post thaw, reduce to 10% FBS once the cells have recovered.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

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