2nd Gen. Packaging Mix & Lentifectin Combo Pack

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LV003-G074Kit
$380.00

Customize 2nd Gen. Packaging Mix & Lentifectin Combo Pack
2nd Generation Packaging System Mix   + $0.00
Lentifectin™ Transfection Reagent   + $0.00

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2nd Gen. Packaging Mix & Lentifectin Combo Pack
2nd Gen. Packaging Mix & Lentifectin Combo Pack

In stock

$380.00

Summary
    Specifications


    Description

    For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest (cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a wildtype 5' LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd lentiviral expression vector with a chimeric 5' LTR. All the lentiviral expression vectors marketed by ABM are 3rd generation with a chimeric of RSV promoter upstream of 5'-LTR.

    SKULV003-G074
    Unit quantityKit
    CautionNot for diagnostic use.
    Note

    NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only.

    Vector Map

    pLenti-P2A, pLenti-P2B

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    References


    1
    • Von Dwingelo, J., Chung, I. Y. W., Price, C. T., Li, L., Jones, S., Cygler, M., & Abu Kwaik, Y. "Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex" mBio 10(4): (2019). DOI: 10.1128/mbio.01942-19.