miRNA Mimic Negative Control

Cat. No.
MCH00000
Unit
2.5 nmol
Price
$45.00

Lead Time
3 to 4 weeks
Cat. No. MCH00000
Name miRNA Mimic Negative Control
Unit 2.5 nmol
Description

miRNA Mimic Negative Control are validated random sequences which have been tested on mammalian cells and tissues, and are shown to produce no identifiable effects on known miRNA function. Highly recommended for use as negative control for all miRNA mimic experiments.

System Synthetic miRNA
Reconstitution We recommend re-suspending the lyophilized synthetic miRNA using DNase and RNase-free ddH2O. To make a 100 µM stock, dissolve the lyophilized powder using 25 µl of ddH2O (for mimics and agomirs, 2.5 nmol per tube) or 50 µl of ddH2O (for inhibitors and antagomirs, 5 nmol per tube).
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. MCH00000
Print & Download Datasheet
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  • Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., … Chen, Y. "NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer" Experimental Cell Research 374(1):181–188 (2019). DOI: 10.1016/j.yexcr.2018.11.022.
  • Shi J, Gao X, Tian T, et al. Structural basis of Q-dependent transcription antitermination. Nat Commun. 2019;10(1):2925. Published 2019 Jul 2. doi:10.1038/s41467-019-10958-8.
  • Trachman, R., & Ferré-D'Amaré, A. (2019). Tracking RNA with light: Selection, structure, and design of fluorescence turn-on RNA aptamers. Quarterly Reviews of Biophysics, 52, E8. doi:10.1017/S0033583519000064.
  • Alexis Autour, Sunny C. Y. Jeng, Adam D. Cawte, Amir Abdolahzadeh, Angela Galli, Shanker S. S. Panchapakesan, David Rueda, Michael Ryckelynck & Peter J. Unrau "Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells" Nature Communications. 2018 Feb;23(9):656. DOI: 10.1038/s41467-018-02993-8.
  • Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al. "Ribonucleoprotein purification and characterization using RNA Mango." RNA. 2017 Oct;23(10):1592-1599. DOI: 10.1261/rna.062166.117.
  • Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D'Amaré AR. et al. "Structural basis for high-affinity fluorophore binding and activation by RNA Mango." Nat Chem Biol. 2017 Jul;13(7):807-813. DOI: 10.1038/nchembio.2392.
  • Cawte, Adam. et al. "Live Cell Imaging of Genomic Loci using Fluorescent RNA Aptamers." Biophysical Journal. 2017 Feb, Volume 112,Issue 3,69a. DOI: 10.1016/j.bpj.2016.11.416.
  • Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al. "Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers." RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24. PubMed : 27777365.
  • Ouellet J. et al. "RNA Fluorescence with Light-Up Aptamers." Front Chem. 2016 Jun 28;4:29. DOI: 10.3389/fchem.2016.00029. eCollection 2016. Review. PubMed : 27446908.
  • Panchapakesan SS, Jeng SC, Unrau PJ. et al. "RNA complex purification using high-affinity fluorescent RNA aptamer tags." Ann N Y Acad Sci. 2015 Apr;1341:149-55. DOI: 10.1111/nyas.12663. Epub 2015 Jan 13.
  • Dolgosheina EV, Jeng SC, Panchapakesan SS, Cojocaru R, Chen PS, Wilson PD, Hawkins N, Wiggins PA, Unrau PJ. et al. "RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking." ACS Chem Biol. 2014 Oct 17;9(10):2412-20. DOI: 10.1021/cb500499x. Epub 2014 Aug 21.
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