RAD51C Lentiviral Vector / Virus (Human)

Datasheet SDS

  • Retired Cat.No.

    38423061

  • Product Name

    RAD51C Lentiviral Vector (Human)

  • Unit

    1.0 µg DNA

  • Description

    Save 30% OFF Packaging Mix or Transduction Enhancer with any lentivector or lentivirus. Click here to claim your savings!
    The RAD51C expression will be driven by a CMV promoter, without any Tag or reporter. Inserts are flanked by and can be excised using NheI and XhoI as long as inserts do not contain any internal NheI or XhoI sites.

  • Gene Name/Gene ID

    RAD51C, -2

  • Gene Full Name

    RAD51 homolog C (S. cerevisiae)

  • Alias

    RAD51L2

  • Accession Number

    NM_058216.3

  • Species

    Human

  • System

    Lentiviral Vector

  • Promoter

    CMV

  • Insert Size

    1131

  • Vector Size

    7684

  • Storage Condition

    Store Lentiviral Vectors at -20°C.

  • Disclaimer

    Click for more info

  • Caution

    This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
    Publishing research using LC138423? Please let us know so that we can cite the reference in this datasheet.
    LC138423 has not been cited in any literature.
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    Question
    How should I store my lentivirus?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    The lentivirus should be aliquoted into smaller working volumes and then stored at -80°C. Lentivirus is sensitive to storage temperature and to freeze/thaws. It can lose up to 5% or more activity with each freeze/thaw. When stored properly, viral stocks of an appropriate titer should be suitable for use for up to one year. After long-term storage, we recommend re-titering your viral stocks before use.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    What generation does abm use for their lentiviral transfer vectors?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    Is abm’s lentivirus system Tat dependent or independent?
    ABM community
    Verified customer
    Asked on Mar 02 2025
    Answer
    Tat-Independent.
    ABM Scientific Support
    Answered on Mar 02 2025
    Question
    How do I know if abm’s lentivirus is replication defective?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Lentiviral vector stocks are tested for the presence of replication-competent lentivirus (RCL) by monitoring p24 antigen expression in the culture medium of transduced 293T cells for 30 days. Since the vectors are replication-defective, no amplification of the p24 expression would normally be expected. However, if there were an increase in P24 signal over time, it would be indicative of RCL contamination. The p24 expression is assayed by qPCR.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    How efficient is lentivirus transduction?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Transduction is highly dependent on the cell line or cell type. In general, lentivirus has high transduction efficiency in most cell lines with up to 100% efficiency in HEK293 and other commonly used cell lines. The lowest efficiency observed is 10%. We recommend customers evaluate transduction efficiency for a specific cell line by using a control GFP lentivirus. For customers that aim to generate a stable cell line, 1% transduction efficiency is enough as every transduced cell is permanently integrated with the vector. Therefore, 10ml of 10^6 cfu/ml lentivirus would result in more than enough transduced cells.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    Are abm’s lentiviral vectors compatible with my packaging mix?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    Following lentivirus infection into my target cells I noticed that the cell’s have a shorter life span compared to my uninfected cells. Why?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The supernatant is collected from the viral production stage and this is used to infect the target cells. DMEM media is utilized during production and often at the time of harvesting it has been depleted of nutrients. Because of this the supernatant may create a stressful environment for the target cells or DMEM may be unsuitable for your cell line.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    Does MOI affect antibiotic resistance?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    How do I calculate the Multiplicity of Infection (MOI)?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.

    MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    What concentration of puromycin should I use for cell selection following lentivirus transduction?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    The concentration must be optimized for each cell type.  Typical selection amounts are around 0.1 - 0.5µg per ml.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    Are abm’s lentiviral vectors compatible with packaging plamids psPAX2 and pDM2.G?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Yes.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    What is the optimal cell density for lentivirus transduction?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    Optimal cell density for lentiviral transduction is generally 50-70% confluent for most cell types. Certain cell lines may require the optimal cell density to be determined experimentally.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    Does vector pLenti-GIII-CMV contain recombination sites flanking the insert sequence?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Yes, there is an extra ~125bp to either side of the CDS insert. Thus, if the vector is digested using EcoRV restriction enzyme the resulting DNA band will be the insert size plus 250bp.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    We want to study promoter function using a lentiviral vector. If the 5’ LTR already contains a promoter, would it interfere with our downstream promoter?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    If your promoter is over 500bp, it is less likely to see any effect from the LTR.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    Are abm’s vectors high or low copy? What is the expected yield from a plasmid extraction prep?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    Some of abm’s vectors contain the tet operator site (tetO). Does this mean that gene expression is inducible?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The tetO element is essentially residual DNA sequence originating from the backbone cloning vector. The tetO sequence will play no role in repression/induction in the absence of the appropriate tet promoter, tet repressor (TetR) and doxycycline.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    Some of abm’s vectors contain a C-terminal HA tag. Does this mean that my gene has an HA tag?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The HA tag is residual DNA sequence originating from the backbone cloning vector. The HA tag will not be expressed because the GOI will have an upstream stop codon.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    Do abm’s lentiviral vectors contain a chimeric RSV promoter in the upstream 5’ LTR?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Yes.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    How are abm’s vectors shipped and what buffer are they supplied in?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    Standard delivery of abm’s vectors are in liquid format supplied in TE buffer (10mM Tris, 1mM EDTA, pH 8.0). Our vectors can also be ordered and delivered in agar stabs for an additional fee.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    Do you recommend using 2nd or 3rd generation lentivirus packaging mix?
    ABM community
    Verified customer
    Asked on Aug 08 2025
    Answer
    abm's lentiviral vectors are compatible with 2nd and 3rd generation packaging systems. We recommend abm’s Second Generation Packaging Mix (Cat. No. LV003) for users who want to achieve higher titer lentivirus as only 3 plasmids are required for virus production instead of 4 plasmids.  We also recommend abm’s Third Generation Packaging Mix (Cat. No. LV053) for users concerned with virus biosafety, as the viral packaging elements are further split into 4 plasmids thus reducing probably of producing wild type virus.

    Please note, only packaging mixes produced by abm have been tested in house and therefore carry our guarantee for high titer virus production. If it is desirable to use other packaging plasmids obtained from a different source, the compatibility must be tested and determined by the end user.
    ABM Scientific Support
    Answered on Aug 08 2025
    Question
    Can I insert my own polyA signal into abm’s lentiviral vector? Where is the polyA signal located in abm’s lentiviral vectors?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Since lentivirus is an RNA virus, during the synthesis of the RNA genome to be packaged, if there is a polyadenylation signal in the insert, the RNA will be terminated prematurely. There is a SV40 poly(A) signal in the vector located downstream of the 3'LTR. It is designed this way to ensure a high amount of transcriptional RNA is present so that a high viral titer is obtained. We have received positive feedback from previous customers in regards to the high and stable expression levels of our lentivectors.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    What concentration are abm’s vectors supplied as?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    Typically vectors are supplied at 100ng/µl.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    Is a kozak sequence required for mammalian gene expression?
    ABM community
    Verified customer
    Asked on Nov 13 2025
    Answer
    Yes, a Kozak sequence (GCCGCCACCATGG) immediately upstream of the ATG is required to ensure optimal protein translation. This is true for all mammalian expression vectors that we offer. Please note that blank vector/viruses do not express anything so the Kozak sequence will be absent.
    ABM Scientific Support
    Answered on Nov 13 2025
    Question
    What makes abm’s lentivirus replication deficient?
    ABM community
    Verified customer
    Asked on Jan 28 2025
    Answer
    The lentivirus system is based on the inactivated HIV genome. The gag, pol, rev, env, vif, vpr, tat, vpu, and nef elements involved in lentiviral replication are not present in our lentiviral expression vectors.
    ABM Scientific Support
    Answered on Jan 28 2025
    Question
    Do abm’s lentiviral vectors contain the WPRE?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Yes, all of abm’s lentiviral vectors contain the WPRE (woodchuck hepatitis virus post-transcriptional regulatory element) upstream of the 3’LTR. This element when transcribed creates a tertiary structure which enhances gene expression.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    How should I store abm’s vectors?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    We recommend storing abm’s vectors at -20°C and avoiding repeated freeze/thaws as this may affect plasmid integrity and cloning efficiency.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    What is MOI and how do I know which MOI to use?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    What buffer is abm’s lentivirus provided in?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    10^7 IU/ml = DMEM
    10^8, 10^9, 10^10 IU/ml = 1X PBS (pH 7.4) with 2% PEG6000
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    How do I transform abm’s plasmids? What bacterial strain should I use?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    A general Plasmid Amplification Protocol can be found here.

    We recommend transforming abm’s plasmids into our ProClone™ Competent Cells (Cat. No. E003). The ProClone™ Competent Cells are high-efficiency chemically competent DH5α (E. coli) cells ideal for routine plasmid amplification due to its high yield, rapid growth, and high transformation efficiency, with recA⁻ and endA⁻ mutations that ensure plasmid stability and high DNA quality. Other common compatible cloning strains include TOP10 (DH10B derivatives).
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    How do I revive an agar stab?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    1. Use a sterile loop or pipette tip to scrape a small amount of cells from the agar stab. Only a tiny amount is needed as it contains live E. coli.
    2. Gently streak the cells onto an agar plate containing the correct antibiotic selection in order to achieve single isolated colonies.
    3. Place the plate “agar side up” and incubate at 37°C overnight.
    4. Select a single colony from the plate for downstream applications, such as an overnight broth culture and grow E. coli to late exponential-early stationary phase. 4a. The broth culture can be subjected to miniprep plasmid extraction. We recommend using abm’s Column-Pure Plasmid Miniprep Kit (Cat. No. G4003). 4b. The broth culture can be used to prepare a glycerol stock for long-term storage. Mix culture with sterile glycerol to a final concentration of 15% and store at -80°C.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    During lentivirus production, does only the gene of interest get incorporated into the capsid of lentivirus?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    During the process of lentivirus packaging, the genes (including the gene of interest) between the 5'-LTR and 3'-LTR of the transfer plasmid (i.e. lentivector DNA) will be incorporated into the packaged recombinant lentivius. The packaging plasmids are provided separately from the transfer plasmid, and their purpose is to provide pseudotyping and structural protein instructions only. They will not be incorporate into the capsid.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    I packaged abm’s lentivector into virus, performed transduction and antibiotic selection. Following selection all my cells died. What should I do?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    We recommend first checking the following parameters:
    1. Cell density. If density is too low, this can also result in cell death.
    We recommend the following experiments:
    Initial Experiment
    •Transduce lentivirus into the 293T cell line - one of the easiest cell lines to transduce. If the transduced cells survive selection, then troubleshooting should focus on post-virus production steps. If the transduced cells didn't survive selection, the troubleshooting should focus on virus packaging steps.
    Virus Packaging Troubleshooting
    •Attempt lentivirus packaging using a GFP expressing control vector. If packaging is successful with the control, then repeat packing of the lentivirus in question with a GFP batch control to ensure no errors with the first production. If no GFP signal is observed after transfection or ~72h after a test transduction of a GFP control lentivirus on 293T cells, then the packaging process needs to be assessed.  Packaging process assessment includes reagent quality, cell health, and packaging protocol details.
    Post-Virus Production Troubleshooting
    •Assess if the correct antibiotic concentration is used. This can be done by performing a drug-killing curve. To set up a drug-killing curve, we recommend using the same culture size and seeding density for your actual selection, and adding a different puromycin concentration to each sample, with the range between 0-1 ug/ml. If the cells are not killed at the 1ug/ml, you may try increasing the range higher concentrations. It is important to identify the concentration that results in >95% cell death in 1-4 days to establish the minimal concentration to use for the selection process.
    •Assess if the correct MOI is used to transduce the target cells. This can be done by using a GFP control lentivirus to transduce the target cells at a range around literature-recommended MOIs to determine the optimal MOI for transduction.
    •Assess if a transduction enhancer such as the ViralEntry Transduction Enhancer (Cat.No. G515) is necessary. Some cell lines are more difficult to transduce than others and by using a transduction enhancer can lead to increased target cell permeability.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    I transduced my cell line using abm’s virus. Afterwards my cells exhibited a different morphology and/or died. Why?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    1. This often happens to primary cells which are very sensitive to culture medium conditions. Try to use a higher titer virus in order to limit the volume of exogenous media added to your cells.
    2. Virus preparation might be contaminated with bacteria. Use a 0.25µm syringe filter to clear the virus preparation and repeat transduction following strict sterile technique.
    3. Cell line might be contaminated with mycoplasm. This type of contamination is often not immediately obvious. The contamination effect is amplified after virus transduction. Repeat transduction with fresh cells.
    4. The virus preparation may contain some cell debris which could be mistaken as a change in cell morphology. Normally this issue will disappear 3-5 days after virus transduction.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    I am interested in one of abm’s vectors. Where can I find the corresponding control?
    ABM community
    Verified customer
    Asked on Jan 30 2026
    Answer
    A complete list of controls can be found on abm’s Control Vectors and Viruses page.
    ABM Scientific Support
    Answered on Jan 30 2026
    Question
    I used abm’s virus to transduce my cell line. I determined that there is mRNA expression of my GOI but no protein expression. Why?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    There could several reasons for this:
    1. qPCR detection is much more sensitive than Western blot, and any target signal is amplified greatly during qPCR.
    2. Verify that your primary antibody is of good quality and include good positive and negative controls in your Western blot.
    3. Western blot can only be performed after a stable cell line has been established and notably it rarely works well with a polyclonal cell culture.
    4. mRNA expression does not always correlate with protein expression because many different biological factors may affect translation, including protein half-life, protein degradation and molecular processes such as phosphorylation, ubiquitination, methylation, etc. abm is not in a position to guarantee GOI expression at the protein level due to the number of experimental variables involved. abm guarantees GOI expression at the mRNA level only, while expression at the protein level must be determined experimentally. We recommend checking the literature to see whether other scientists have been able to over-express the protein in the same target cells and how this was achieved (tag, delivery system, detection method, etc).
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    I transduced abm’s lentivirus into my target cell line but there is no fluorescence or very weak signal. Why?
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    There could many possible reasons for this result.
    1. Typically GFP is behind a weaker promoter such as SV40 or CBH leading to a less robust fluorescence signal.
    2. Virus titer is low leading to only a small proportion of successfully transduced cells. Try increasing the MOI or using a transduction enhancer such as abm’s ViralEntry™ (Cat.No. G516). 3. Target cells are difficult to transduce. A higher MOI must be tested. Some cells are very easy to be transduced such as 293T, while others such as lymphocytes are very difficult.
    4. Lentivirus became inactivated during transportation and storage. Lentivirus is very sensitive to temperature changes. Leaving lentivirus at room temperature, 4°C for 48 hours, or subjecting it to multiple freeze/thaws can lead to inactivation or reduced titer.
    ABM Scientific Support
    Answered on Mar 24 2025
    Question
    What are the advantages and disadvantages of different viral systems (AAV/Lentivirus/Adenovirus/ Retrovirus)?
    ABM community
    Verified customer
    Asked on Jan 16 2026
    Answer
    Each viral vector system has different strengths depending on the application:
    The best choice depends on target cells, expression duration, payload size, and safety requirements.
    ABM Scientific Support
    Answered on Jan 16 2026
    Question
    How do I determine which viral vector to use for my experiment?
    ABM community
    Verified customer
    Asked on Jan 16 2026
    Answer
    Vector selection depends on several practical factors:
    If customers are unsure, we recommend sharing target cell type, payload size, and desired expression duration so we can suggest the most appropriate approach.
    ABM Scientific Support
    Answered on Jan 16 2026
    Question
    What troubleshooting steps do you recommend if plasmid amplification fails?
    ABM community
    Verified customer
    Asked on Jan 29 2026
    Answer

    • Verify plasmid quality: Confirm the plasmid is intact, at sufficient concentration, and free of contaminants (e.g., salts, ethanol, nucleases).

    • Check the host strain: Use a suitable cloning strain (e.g., recA⁻/endA⁻ such as DH5α) and confirm cells are competent and not expired.

    • Confirm selection conditions: Make sure the correct antibiotic is used at the proper concentration and that plates/media are fresh.

    • Review transformation conditions: Ensure the transformation method (chemical or electrocompetent) and recovery time are appropriate.

    • Assess plasmid compatibility: Large, low-copy, toxic, or unstable plasmids may require specialized strains or growth conditions.

    • Inspect growth conditions: Verify incubation temperature, shaking speed, and culture time.

    • Check plasmid sequence/features: Look for toxic genes, strong promoters, or repeats that may reduce stability.

    ABM Scientific Support
    Answered on Jan 29 2026
    Question
    After introducing my over-expression vector/virus into my cell line I did not observe over expression of my gene of interest. What troubleshooting steps do you recommend?
    ABM community
    Verified customer
    Asked on Jan 29 2026
    Answer

    • Confirm vector delivery: Verify successful transfection or transduction using a reporter, marker, or control vector.

    • Check expression at multiple levels: Confirm expression at the RNA level (e.g., transcript detection) as well as protein level, since post-transcriptional regulation may occur.

    • Consider cell type effects: CMV promoter activity can vary between cell lines and may be weak or silenced in certain cells.

    • Evaluate gene-specific factors: Some genes are toxic, unstable, or tightly regulated, which can reduce detectable overexpression.

    • Review culture and timing: Expression levels may depend on cell health, passage number, and time post-delivery.

    • Validate construct design: Ensure the insert is in the correct orientation, reading frame, and matches the expected sequence.

    ABM Scientific Support
    Answered on Jan 29 2026
    Question
    After introducing my over-expression vector/virus into my cell line, I performed Western blot but the detected molecular weight differs from the expected size. Why is this and what troubleshooting steps should I take?
    ABM community
    Verified customer
    Asked on Jan 29 2026
    Answer

    Differences between the observed and expected molecular weight are relatively common and can result from several factors, including alternative splicing, post-translational modifications, proteolytic processing, or the presence of fusion tags. In some cases, the antibody may also recognize non-specific proteins.

    Basic troubleshooting steps include:

    • Confirming the predicted protein size, including any tags or signal peptides.

    • Verifying antibody specificity and reviewing the datasheet for known cross-reactivity.

    • Checking whether the protein undergoes cleavage or modification in your cell type.

    • Comparing expression to a positive control or reference sample.

    • Confirming construct sequence and reading frame.

    ABM Scientific Support
    Answered on Jan 29 2026
    Question
    After introducing my over-expression vector/virus into my cell line, no GFP expression is observed, even at high MOI. Why is this and what troubleshooting steps should I take?
    ABM community
    Verified customer
    Asked on Jan 29 2026
    Answer

    Lack of GFP expression can occur even when delivery appears successful. Expression levels may be influenced by cell type, promoter activity, transduction efficiency, or cellular stress. In some cells, CMV or other strong promoters may be silenced, and high MOI can negatively impact cell health.

    Basic troubleshooting steps include:

    • Confirming successful delivery using an alternative marker or control construct.

    • Assessing cell viability and morphology after transduction.

    • Allowing sufficient time post-delivery for expression.

    • Evaluating whether the promoter is active in your specific cell line.

    • Verifying construct integrity and GFP coding sequence.

    Because expression outcomes are highly system-dependent, optimization may be required for each experimental setup.

    ABM Scientific Support
    Answered on Jan 29 2026
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Current vector selected:
RAD51C Lentiviral Vector (Human)
Cat. No.
LC138423
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Plasmid DNA Services
Midiprep Plasmid Amplification
10-20 µg
$75.00
Maxiprep Plasmid Amplification
80-100 µg
$150.00
Bacterial Agar Stab
1 stab
$40.00
Virus Packaging Services
Lentivirus packaging at 108 IU/ml Titer (Mini)
3 x 250 µl
$189.00
Lentivirus packaging at 109 IU/ml Titer (Regular)
4 x 100 µl
$479.00
Lentivirus packaging at 1010 IU/ml Titer (Ultra-Pure)
10 x 50 µl
$1,199.00
Controls and Related Products
Lenti-CMV-Blank-SV40-Puro Control Vector
LV587
1.0 µg
$99.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
qPCR Lentivirus Titer Kit
LV900
100 rxn
$212.00
2nd Generation Packaging Mix
LV003
200 µl
$293.00
3rd Generation Packaging Mix
LV053
200 µl
$355.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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Current vector selected:
Cat. No.
Controls and Related Products
Lenti-CMV-Blank-SV40-Puro Control Vector
LV587
1.0 µg
$99.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
qPCR Lentivirus Titer Kit
LV900
100 rxn
$212.00
2nd Generation Packaging Mix
LV003
200 µl
$293.00
3rd Generation Packaging Mix
LV053
200 µl
$355.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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RAD51C Lentiviral Vector (Human)
LC138423
Lentivirus Packaging at 107 IU/ml Titer
LV7-LC138423
Lentivirus Packaging at 108 IU/ml Titer
LV8-LC138423
Lentivirus Packaging at 109 IU/ml Titer
LV9-LC138423
Lentivirus Packaging at 1010 IU/ml Titer (Ultra-pure)
LV10-LC138423
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